Identification of Severely Burned Bodies From a Vehicle Collision Using the Restriction Fragment Length Polymorphism (RFLP) Technique: Figures, by Lim, Jaya, Kassim, Seah, Lee, and Chee (Forensic Science Communications, July 2000)
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July 2000 - Volume 2 - Number 3 |
Case Report: Identification of Severely Burned Bodies From a Vehicle Collision Using the Restriction Fragment Length Polymorphism (RFLP) Technique
Figure 1
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* Single allele (homozygote) |
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Figure 2
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* Either of these two alleles (1928 bp and 1035 bp; 2905 bp and 1165 bp) could have been inherited from the father. No blood sample from the mother was available for comparison. + The allele, 3230 bp at genetic locus D4S139 (PH30), obtained from Body 6 does not match that of the father, 3432 bp (difference = 6.2 percent), but the other fragment allele, 5131 bp, matches with that of the brother, 5174 bp (maternal allele). This could be due to mutation. |
Figure 3
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The alleles inherited from the father were determined (from the alleles of his three children) to be as follows,
where + indicates that the allele obtained is from the average of all the matching alleles and ? could be 2554 bp (i.e., homozygote) or another allele not detected in any of the three children. |
Figure 4
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* Either of these two alleles could have been inherited from the father. No blood sample from the mother was available for comparison. —— No DNA profile at this genetic locus was obtained because of insufficient DNA. |
Figure 5
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* Either of these two alleles could have been inherited from the mother (Body 11). No blood sample from the father (son-in-law) was available for comparison. ** From the results, Son 2 and Body 7 (Daughter 2) were found to be the biological children of the mother from Family 5, but not of the father (Body 10). However, both Son 2 and Body 7 (Daughter 2) have matching paternal alleles at genetic loci D4S139 (PH30, 5881 bp and 5841 bp) and D5S110 (LH1, 4497 bp and 4583 bp), while the allele at D4S139 also matches that of the father, Body 10 (PH30, 6034 bp). In addition, Daughter 2 has another allele at genetic locus D10S28 (TBQ7, 1015 bp) that matches that of the father (968 bp). All these indicate that both the Son 2 and Daughter 2 are likely to have the same biological father, who could be related to the father of Family 5 (Body 10). |
Figure 6
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* Either of these two alleles (2088 bp and 1207 bp; 1921 bp and 1726 bp) at genetic loci D10S28 (TBQ7) and D2S44 (YNH24), respectively, could have been inherited from the mother (Body 3). No blood sample from the father was available for comparison. |