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Forensic Mitochondrial DNA Analysis (DNA 170)

Course Length: Two weeks. Eligible Students: Crime laboratory examiners and DNA biologists conducting or preparing to conduct forensic mitochondrial DNA (mtDNA) analysis. Description: This course provides classroom and laboratory experience in the principles and procedures involved in typing mtDNA from evidentiary items such as hair, teeth, and bones. Classroom instruction is focused on the nature of mtDNA, molecular biology principles involved in the polymerase chain reaction (PCR) and sequencing, and legal issues related to this technique. Discussions regarding scientific literature published in the area of forensic mtDNA analysis will also be conducted. Laboratory procedures include DNA extraction, PCR, quantification of amplified products by capillary electrophoresis, and automated sequencing. Computer-based practice compiling sequences and database searches using appropriate software is provided. Moot court exercises are also conducted.

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