Figure 4 is a bar graph that summarizes the proportion of successfully typed DNA profiles from the three different DNA extraction methods. The proportion of typed allelic peaks from the three different DNA extraction methods for the 12 loci of the AmpFℓSTR SEfiler PCR Amplification Kit are arranged according to increasing fragment lengths in base pairs. The graph shows the average values for the heterozygous loci and the half of the values of the homozygous loci. The average relative fluorescent unit values from the three different DNA extractions and the standard deviations were also plotted.

The multiplex PCR system showed that the DNA extracted by the silica-based method and that extracted by nexttec columns resulted in the same allelic characterization. The nexttec extraction method with a fill height of 14 mm led to the strongest amplification. The increase in the peak heights compared to the Qiagen method is probably due to the higher amount of DNA in the amplification setup. The increase in the peak heights of the nexttec column with a fill height of 14 mm compared to the nexttec column with a fill height of 7 mm might be caused by a higher quality of the DNA. Overall, the data show that the nexttec extraction method with a fill height of 14 mm may be more efficient in removing inhibitors without reducing the amount of recovered DNA.

A New Approach for the Extraction of DNA from Postage Stamps Page