Dilution

OneStep ABA Cardâ p30

w/water

Seratecâ PSA Semiquant #1

w/water

Seratecâ PSA Semiquant #2

w/water

Seratecâ PSA Semiquant #1

w/HEPES

Seratecâ PSA Semiquant #2

W/HEPES

Straight Semen

+

+   T > q

+   T > q

+   T > q

+   T > q

1:10

+

+   T > q

+   T > q

+   T > q

+   T > q

1:50

+

+   T = Q

+   T = Q

+   T > q

+   T > q

1:100

-

+   t < Q

+   t < Q

+   T > q

+   T > q

1:250

-

+   t < Q

+   t < Q

+   t < Q

+   t < Q

1:500

-

+   t < Q

+   t < Q

+   t < Q

+   t < Q

1:1,000

-

+   t < Q

+   t < Q

+   t < Q

-

1:2,500

-

-

+   t < Q

-

-

1:5,000

-

-

-

-

-

1:10,000

-

-

-

-

-

Negative Control (sterile water or HEPES only)

-

-

-

-

-