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Identification of Severely Burned Bodies From a Vehicle Collision Using the Restriction Fragment Length Polymorphism (RFLP) Technique: Figures, by Lim, Jaya, Kassim, Seah, Lee, and Chee (Forensic Science Communications, July 2000)
- U.S. Department of Justice
- Federal Bureau of Investigation
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July 2000 Volume 2 Number
3
Case Report:
Identification of Severely Burned Bodies From a Vehicle Collision
Using the Restriction Fragment Length Polymorphism (RFLP) Technique
Figure 1
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Figure 1. Family
1 is composed of one of the victims (Body 2) and three next of
kin (mother, father, and sister). Alleles inherited from the
father are underlined. Alleles inherited from the mother are
in italics.
* Single allele (homozygote)
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Figure 2
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Figure 2. Family
2 is composed of one of the victims (Body 6) and two next of
kin (father and brother). Alleles inherited from the father are
underlined. Alleles inherited from the mother are in italics.
* Either of these two alleles
(1928 bp and 1035 bp; 2905 bp and 1165 bp) could have been inherited
from the father. No blood sample from the mother was available
for comparison.
+ The allele, 3230 bp at
genetic locus D4S139 (PH30), obtained from Body 6 does not match
that of the father, 3432 bp (difference = 6.2 percent), but the
other fragment allele, 5131 bp, matches with that of the brother,
5174 bp (maternal allele). This could be due to mutation.
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Figure 3
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Figure 3. Family 3 is composed of one of the
victims (Body 5) and four next of kin (brother, daughter, and
two sons). Alleles inherited from the father are underlined.
Alleles inherited from the mother are in italics.
The alleles inherited from
the father were determined (from the alleles of his three children)
to be as follows,
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YNH24 |
PH30 |
LH1 |
TBQ7 |
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2346 |
4828 |
2554+ |
2876 |
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1547+ |
4175+ |
? |
953+ |
where + indicates that the
allele obtained is from the average of all the matching alleles
and ? could be 2554 bp (i.e., homozygote) or another allele not
detected in any of the three children.
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Figure
4
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Figure 4. Family
4 is composed of three victims (Bodies 1, 8, and 13) and three
next of kin (daughter, son-in-law, and son of victim [Body 13]).
Alleles inherited from the father are underlined. Alleles inherited
from the mother are in italics. Alleles in boldface are those
inherited from the son-in-law (i.e., the father of the twin daughters
[Bodies 1 and 8]).
* Either of these two alleles
could have been inherited from the father. No blood sample from
the mother was available for comparison.
---- No DNA profile at this
genetic locus was obtained because of insufficient DNA.
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Figure 5
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Figure 5. Family
5 is composed of three victims (Bodies 7, 10, and 11) and four
next of kin (wife, two sons, and grandson of victim [Body 10]).
Alleles inherited from the father are underlined. Alleles inherited
from the mother are in italics. Alleles in boldface are those
inherited from the son-in-law (i.e., the father of the grandson).
* Either of these two alleles
could have been inherited from the mother (Body 11). No blood
sample from the father (son-in-law) was available for comparison.
** From the results, Son
2 and Body 7 (Daughter 2) were found to be the biological children
of the mother from Family 5, but not of the father (Body 10).
However, both Son 2 and Body 7 (Daughter 2) have matching paternal
alleles at genetic loci D4S139 (PH30, 5881 bp and 5841 bp) and
D5S110 (LH1, 4497 bp and 4583 bp), while the allele at D4S139
also matches that of the father, Body 10 (PH30, 6034 bp). In
addition, Daughter 2 has another allele at genetic locus D10S28
(TBQ7, 1015 bp) that matches that of the father (968 bp). All
these indicate that both the Son 2 and Daughter 2 are likely
to have the same biological father, who could be related to the
father of Family 5 (Body 10).
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Figure 6
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Figure 6. Family 6 is composed of four victims
(Bodies 3, 4, 9, and 12) and two next of kin (son of victim [Body
3] and sister of victim [Body 12]). Alleles inherited from the
father are underlined. Alleles inherited from the mother are
in italics. Alleles in boldface are those inherited from the
son-in-law (the father of the grandson [Body 4]).
* Either of these two alleles
(2088 bp and 1207 bp; 1921 bp and 1726 bp) at genetic loci D10S28
(TBQ7) and D2S44 (YNH24), respectively, could have been inherited
from the mother (Body 3). No blood sample from the father was
available for comparison.
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FORENSIC SCIENCE COMMUNICATIONS JULY 2000 VOLUME
2 NUMBER 3 |
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