Home About Us Laboratory Services Forensic Science Communications Back Issues July 2000 Identification of Severely Burned Bodies From a Vehicle...

Identification of Severely Burned Bodies From a Vehicle Collision Using the Restriction Fragment Length Polymorphism (RFLP) Technique: Figures, by Lim, Jaya, Kassim, Seah, Lee, and Chee (Forensic Science Communications, July 2000)

    U.S. Department of Justice
    Federal Bureau of Investigation
 FBI Seal graphic

Layout graphic: Topline

 

 

 

Graphic link to the Current Issue's table of contents

Graphic link to the Back Issues page with links to each issue

Graphic link to the Search page

Graphic link to the Journal Description page

Graphic link to the Editors page

Graphic link to the Instructions for Authors

Graphic link to the Handbook of Forensic Services

Graphic link to the Links page

Graphic link to the FBI Lab Home Page

Graphic link to FBI Publications page (publications listing)

 

 

 

Sidebar graphic

Masthead - Forensic Science Communications
July 2000   Volume 2   Number 3

Case Report: Identification of Severely Burned Bodies From a Vehicle Collision Using the Restriction Fragment Length Polymorphism (RFLP) Technique


Figure 1

Figure 1: Family tree 1


Figure 1.
Family 1 is composed of one of the victims (Body 2) and three next of kin (mother, father, and sister). Alleles inherited from the father are underlined. Alleles inherited from the mother are in italics.

* Single allele (homozygote)

Back to the text


Figure 2

Figure 2: Family tree 2


Figure 2.
Family 2 is composed of one of the victims (Body 6) and two next of kin (father and brother). Alleles inherited from the father are underlined. Alleles inherited from the mother are in italics.

* Either of these two alleles (1928 bp and 1035 bp; 2905 bp and 1165 bp) could have been inherited from the father. No blood sample from the mother was available for comparison.

+ The allele, 3230 bp at genetic locus D4S139 (PH30), obtained from Body 6 does not match that of the father, 3432 bp (difference = 6.2 percent), but the other fragment allele, 5131 bp, matches with that of the brother, 5174 bp (maternal allele). This could be due to mutation.

Back to the text

Figure 3

Figure 3: Family tree 3


Figure 3. Family 3 is composed of one of the victims (Body 5) and four next of kin (brother, daughter, and two sons). Alleles inherited from the father are underlined. Alleles inherited from the mother are in italics.

The alleles inherited from the father were determined (from the alleles of his three children) to be as follows,

 YNH24  PH30  LH1  TBQ7
 2346  4828  2554+  2876
 1547+  4175+  ?  953+

where + indicates that the allele obtained is from the average of all the matching alleles and ? could be 2554 bp (i.e., homozygote) or another allele not detected in any of the three children.

Back to the text

Figure 4

Figure 4: Family tree 4


Figure 4.
Family 4 is composed of three victims (Bodies 1, 8, and 13) and three next of kin (daughter, son-in-law, and son of victim [Body 13]). Alleles inherited from the father are underlined. Alleles inherited from the mother are in italics. Alleles in boldface are those inherited from the son-in-law (i.e., the father of the twin daughters [Bodies 1 and 8]).

* Either of these two alleles could have been inherited from the father. No blood sample from the mother was available for comparison.

---- No DNA profile at this genetic locus was obtained because of insufficient DNA.

Back to the text

Figure 5

Figure 5: Family tree 5


Figure 5.
Family 5 is composed of three victims (Bodies 7, 10, and 11) and four next of kin (wife, two sons, and grandson of victim [Body 10]). Alleles inherited from the father are underlined. Alleles inherited from the mother are in italics. Alleles in boldface are those inherited from the son-in-law (i.e., the father of the grandson).

* Either of these two alleles could have been inherited from the mother (Body 11). No blood sample from the father (son-in-law) was available for comparison.

** From the results, Son 2 and Body 7 (Daughter 2) were found to be the biological children of the mother from Family 5, but not of the father (Body 10). However, both Son 2 and Body 7 (Daughter 2) have matching paternal alleles at genetic loci D4S139 (PH30, 5881 bp and 5841 bp) and D5S110 (LH1, 4497 bp and 4583 bp), while the allele at D4S139 also matches that of the father, Body 10 (PH30, 6034 bp). In addition, Daughter 2 has another allele at genetic locus D10S28 (TBQ7, 1015 bp) that matches that of the father (968 bp). All these indicate that both the Son 2 and Daughter 2 are likely to have the same biological father, who could be related to the father of Family 5 (Body 10).

Back to the text

Figure 6

Figure 6: Family tree 6


Figure 6. Family 6 is composed of four victims (Bodies 3, 4, 9, and 12) and two next of kin (son of victim [Body 3] and sister of victim [Body 12]). Alleles inherited from the father are underlined. Alleles inherited from the mother are in italics. Alleles in boldface are those inherited from the son-in-law (the father of the grandson [Body 4]).

* Either of these two alleles (2088 bp and 1207 bp; 1921 bp and 1726 bp) at genetic loci D10S28 (TBQ7) and D2S44 (YNH24), respectively, could have been inherited from the mother (Body 3). No blood sample from the father was available for comparison.

Back to the text

Back to the top

FORENSIC SCIENCE COMMUNICATIONS    JULY 2000   VOLUME 2   NUMBER 3


CURRENT ISSUE BACK ISSUES SEARCH
ALL ISSUES
JOURNAL
DESCRIPTION
EDITORS

INSTRUCTIONS
FOR AUTHORS

LINKS

HANDBOOK OF
FORENSIC SERVICES

LAB HOME

FBI
PUBLICATIONS