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July 1999 Volume 1 Number
2
Mitochondrial DNA Analysis
at the FBI Laboratory
Figure 6
Capillary Electrophoresis
Capillary electrophoresis
is used to quantify the mtDNA amplified in the PCR process. Each
PCR-amplified sample is run with a 200-bp standard of known concentration.
The peak for the 200-bp standard is shown on the left in this
electropherogram, and the peak for the PCR-amplified DNA is on
the right. The area under the 200-bp standard peak is compared
to the area under the peak of the unknown sample to estimate
the concentration of DNA present in the sample. This estimate
is used to prepare the cycle sequencing reactions.
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FORENSIC SCIENCE COMMUNICATIONS JULY 1999 VOLUME
1 NUMBER 2 |
